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The enantiomers of five amino acids (alanine, valine, threonine, leucine and phenylalanine) could be separated by gas chromatography with optically active (S)-5-isopropyl-N3-phenyl-2-thiohydantoinic stationary phase, which prepared from L-valine and phenylisothiocyanate. Gas chromatographic separations on methylesterificated and N-trifluoroacetylated amino acids have been conducted in isothermal at several column temperatures (180~190, 200, 210oC). The separation factors were 1.29 (alanine, 190oC), 1.35 (valine, 190oC), 1.33 (threonine, 190oC), 1.17 (leucine, 190oC) and 1.05 (phenylalanine, 190oC) and D-isomers eluted prior to L-isomers in every instance. The result of this experiment shows that this stationary phase can be used for the separation of the other amino acids enantiomers.
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